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Formate-induced CO tolerance and methanogenesis inhibition in fermentation of syngas and plant biomass for carboxylate production

Baleeiro, Flávio C. F. ORCID iD icon 1; Varchmin, Lukas 1; Kleinsteuber, Sabine; Sträuber, Heike; Neumann, Anke 1
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)

Abstract:

Background: Production of monocarboxylates using microbial communities is highly dependent on local and degradable biomass feedstocks. Syngas or different mixtures of H$_2$, CO, and CO$_2$ can be sourced from biomass gasification, excess renewable electricity, industrial off-gases, and carbon capture plants and co-fed to a fermenter to alleviate dependence on local biomass. To understand the effects of adding these gases during anaerobic fermentation of plant biomass, a series of batch experiments was carried out with different syngas compositions and corn silage (pH 6.0, 32 °C).
Results: Co-fermentation of syngas with corn silage increased the overall carboxylate yield per gram of volatile solids (VS) by up to 29% (0.47 ± 0.07 g gVS$^{−1}$; in comparison to 0.37 ± 0.02 g gVS$^{−1}$ with a N$_2$/CO$_2$ headspace), despite slowing down biomass degradation. Ethylene and CO exerted a synergistic effect in preventing methanogenesis, leading to net carbon fixation. Less than 12% of the electrons were misrouted to CH$_4$ when either 15 kPa CO or 5 kPa CO + 1.5 kPa ethylene was used. CO increased the selectivity to acetate and propionate, which accounted for 85% (electron equivalents) of all products at 49 kPa CO, by favoring lactic acid bacteria and actinobacteria over n-butyrate and n-caproate producers. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000156325
Veröffentlicht am 01.03.2023
Originalveröffentlichung
DOI: 10.1186/s13068-023-02271-w
Scopus
Zitationen: 1
Web of Science
Zitationen: 1
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Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2023
Sprache Englisch
Identifikator ISSN: 2731-3654, 1754-6834
KITopen-ID: 1000156325
Erschienen in Biotechnology for Biofuels and Bioproducts
Verlag Springer Fachmedien Wiesbaden
Band 16
Seiten 26
Bemerkung zur Veröffentlichung Gefördert durch den KIT-Publikationsfonds
Vorab online veröffentlicht am 17.02.2023
Schlagwörter Mixotrophy, Volatile fatty acids, Medium-chain carboxylic acids, Formic acid, Chain elongation, Microbiome, Carbon monoxide, Ethene, Hexanoic acid, Lactic acid bacteria
Nachgewiesen in Web of Science
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Scopus
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