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Characterization of Systemic Disease Development and Paw Inflammation in a Susceptible Mouse Model of Mayaro Virus Infection and Validation Using X-ray Synchrotron Microtomography

de Carvalho, Ana Carolina; Dias, Carlos Sato B. 1; Coimbra, Laís D.; Rocha, Rebeca P. F.; Borin, Alexandre; Fontoura, Marina A.; Carvalho, Murilo; Proost, Paul; Nogueira, Maurício L.; Consonni, Sílvio R.; Sesti-Costa, Renata; Marques, Rafael Elias
1 Institut für Photonenforschung und Synchrotronstrahlung (IPS), Karlsruher Institut für Technologie (KIT)

Abstract:

Mayaro virus (MAYV) is an emerging arthropod-borne virus endemic in Latin America and the causative agent of arthritogenic febrile disease. Mayaro fever is poorly understood; thus, we established an in vivo model of infection in susceptible type-I interferon receptor-deficient mice (IFNAR−/−) to characterize the disease. MAYV inoculations in the hind paws of IFNAR−/− mice result in visible paw inflammation, evolve into a disseminated infection and involve the activation of immune responses and inflammation. The histological analysis of inflamed paws indicated edema at the dermis and between muscle fibers and ligaments. Paw edema affected multiple tissues and was associated with MAYV replication, the local production of CXCL1 and the recruitment of granulocytes and mononuclear leukocytes to muscle. We developed a semi-automated X-ray microtomography method to visualize both soft tissue and bone, allowing for the quantification of MAYV-induced paw edema in 3D with a voxel size of 69 µm3. The results confirmed early edema onset and spreading through multiple tissues in inoculated paws. In conclusion, we detailed features of MAYV-induced systemic disease and the manifestation of paw edema in a mouse model extensively used to study infection with alphaviruses. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000156500
Veröffentlicht am 06.03.2023
Originalveröffentlichung
DOI: 10.3390/ijms24054799
Scopus
Zitationen: 2
Web of Science
Zitationen: 2
Dimensions
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2023
Sprache Englisch
Identifikator ISSN: 1422-0067
KITopen-ID: 1000156500
HGF-Programm 56.13.11 (POF IV, LK 01) Building Blocks of Life: Structure and Function
Erschienen in International Journal of Molecular Sciences
Verlag MDPI
Band 24
Heft 5
Seiten Art.-Nr.: 4799
Vorab online veröffentlicht am 02.03.2023
Schlagwörter mayaro virus; alphavirus; innate immunity; inflammation; microtomography; synchrotron technology
Nachgewiesen in Dimensions
Web of Science
Scopus
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