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Phenotypic anchoring for OMICS-guided assessment of liver toxicity in zebrafish

Dickmeis, Thomas ORCID iD icon 1; Hayot, Gaelle 1; Clausbruch, Christina Cramer von 1; Martinez Lopez, Ruben; Scholz, Stefan; Colbourne, John; Strähle, Uwe; Weiss, Carsten 1; Peravali, Ravindra 1
1 Institut für Biologische und Chemische Systeme (IBCS), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

The zebrafish (Danio rerio) has become a popular model organism for studying embryonic development, genetics, and toxicology. As vertebrates sharing 70 % of their coding genes with humans, zebrafish are also widely used for modelling human diseases. Their small size, external development, transparent embryos, easy husbandry and breeding, and the large number of eggs they lay make them a valuable model for drug and toxicity screening. Already the older embryos possess a functional liver, allowing to assess compound effects on this organ in vivo.
In the context of PrecisionTox (www.precisiontox.org), a large-scale EU project aiming at OMICS based toxicity prediction using invertebrate and early life stage vertebrate model organisms, we wish to establish a workflow for identifying compounds with adverse effects on the zebrafish embryonic liver.
The selected test compounds are known or suspected liver toxicants. To assess overall toxicity, we used the vibration/startle response of zebrafish embryos as a phenotypic read-out, employing a custom-built system. Embryos were exposed to different concentrations of the compounds for 48 h, and benchmark doses (BMD) were determined using a customized KNIME workflow. ... mehr


Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 03.2023
Sprache Englisch
Identifikator ISSN: 0028-1298, 1432-1912
KITopen-ID: 1000157321
HGF-Programm 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Naunyn-Schmiedeberg's Archives of Pharmacology
Verlag Springer
Band 396
Heft S1
Seiten S61
Bemerkung zur Veröffentlichung Abstract
Vorab online veröffentlicht am 01.03.2023
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