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SMC5/6 complex-mediated SUMOylation stimulates DNA–protein cross-link repair in Arabidopsis

Dvořák Tomaštíková, Eva; Prochazkova, Klara; Yang, Fen; Jemelkova, Jitka; Finke, Andreas; Dorn, Annika 1; Said, Mahmoud; Puchta, Holger 2; Pecinka, Ales
1 Karlsruher Institut für Technologie (KIT)
2 Joseph Gottlieb Kölreuter Institut für Pflanzenwissenschaften (JKIP), Karlsruher Institut für Technologie (KIT)

Abstract:

DNA–protein cross-links (DPCs) are highly toxic DNA lesions consisting of proteins covalently attached to chromosomal DNA. Unrepaired DPCs physically block DNA replication and transcription. Three DPC repair pathways have been identified in Arabidopsis (Arabidopsis thaliana) to date: the endonucleolytic cleavage of DNA by the structure-specific endonuclease MUS81; proteolytic degradation of the crosslinked protein by the metalloprotease WSS1A; and cleavage of the cross-link phosphodiester bonds by the tyrosyl phosphodiesterases TDP1 and TDP2. Here we describe the evolutionary conserved STRUCTURAL MAINTENANCE OF CHROMOSOMEs SMC5/6 complex as a crucial component involved in DPC repair. We identified multiple alleles of the SMC5/6 complex core subunit gene SMC6B via a forward-directed genetic screen designed to identify the factors involved in the repair of DPCs induced by the cytidine analog zebularine. We monitored plant growth and cell death in response to DPC-inducing chemicals, which revealed that the SMC5/6 complex is essential for the repair of several types of DPCs. Genetic interaction and sensitivity assays showed that the SMC5/6 complex works in parallel to the endonucleolytic and proteolytic pathways. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000158082
Veröffentlicht am 21.04.2023
Originalveröffentlichung
DOI: 10.1093/plcell/koad020
Scopus
Zitationen: 1
Web of Science
Zitationen: 2
Dimensions
Zitationen: 3
Cover der Publikation
Zugehörige Institution(en) am KIT Joseph Gottlieb Kölreuter Institut für Pflanzenwissenschaften (JKIP)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2023
Sprache Englisch
Identifikator ISSN: 1040-4651, 1532-298X
KITopen-ID: 1000158082
Erschienen in The Plant Cell
Verlag American Society of Plant Biologists
Band 35
Heft 5
Seiten 1532–1547
Vorab online veröffentlicht am 27.01.2023
Nachgewiesen in Web of Science
Dimensions
Scopus
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