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Activity-enhanced DNAzyme for design of label-free copper( ii ) biosensor

Etheridge, William; Brossard, Frederic; Zheng, Sitan; Moench, Svenja ORCID iD icon 1; Pavagada, Suraj; Owens, Róisín M.; Fruk, Ljiljana
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)

Abstract:

Metal ion-driven, DNA-cleaving DNAzymes are characterised by high selectivity and specificity. However, their use for metal ion sensing remains largely unexplored due to long reaction times and poor reaction yields relative to RNA-cleaving DNAzymes and other sensing strategies. Herein we present a study demonstrating a significant rate enhancement of a copper-selective DNA cleaving DNAzyme by both polydopamine (PDA) and gold (Au) nanoparticles (NPs). PDA NPs enhance the reaction through the production of hydrogen peroxide, while for AuNPs the enhancement is aided by the presence of citrate surface moeities, both of which drive the oxidative cleavage of the substrate. A 50-fold enhancement for PDA NPs makes the combination of PDA and DNAzyme suitable for a practical application as a sensitive biosensor for Cu(II) ions. Using DNAzyme deposition onto a gold electrode followed by Polydopamine Assisted DNA Immobilisation (PADI), we achieve a cost-effective, label-free and fast (within 15 min) electrochemical biosensor with a limit of detection of 180 nmol (11 ppm), thus opening a route for the rational design of a new generation of hybrid DNAzyme-based biosensors.


Verlagsausgabe §
DOI: 10.5445/IR/1000160711
Veröffentlicht am 18.07.2023
Originalveröffentlichung
DOI: 10.1039/d3nr02169f
Scopus
Zitationen: 1
Dimensions
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 07.07.2023
Sprache Englisch
Identifikator ISSN: 2040-3364, 2040-3372
KITopen-ID: 1000160711
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Nanoscale
Verlag Royal Society of Chemistry (RSC)
Band 15
Heft 25
Seiten 10776–10782
Vorab online veröffentlicht am 14.06.2023
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