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An inducible CRISPR‐Kill system for temporally controlled cell type‐specific cell ablation in Arabidopsis thaliana

Gehrke, Fabienne 1; Ruiz-Duarte, Paola; Schindele, Angelina 1; Wolf, Sebastian; Puchta, Holger 1
1 Joseph Gottlieb Kölreuter Institut für Pflanzenwissenschaften (JKIP), Karlsruher Institut für Technologie (KIT)

Abstract:

The application of the CRISPR/Cas system as a biotechnological tool for genome editing has revolutionized plant biology. Recently, the repertoire was expanded by CRISPR-Kill, enabling CRISPR/Cas-mediated tissue engineering through genome elimination by tissue-specific expression.

Using the Cas9 nuclease from Staphylococcus aureus (SaCas9), CRISPR-Kill relies on the induction of multiple double-strand breaks (DSBs) in conserved repetitive genome regions, such as the rDNA, causing cell death of the targeted cells. Here, we show that in addition to spatial control by tissue-specific expression, temporal control of CRISPR-mediated cell death is feasible in Arabidopsis thaliana.

We established a chemically inducible tissue-specific CRISPR-Kill system that allows the simultaneous detection of targeted cells by fluorescence markers. As proof of concept, we were able to eliminate lateral roots and ablate root stem cells. Moreover, using a multi-tissue promoter, we induced targeted cell death at defined time points in different organs at select developmental stages.

Thus, using this system makes it possible to gain new insights into the developmental plasticity of certain cell types. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000161319
Veröffentlicht am 11.08.2023
Originalveröffentlichung
DOI: 10.1111/nph.19102
Scopus
Zitationen: 4
Web of Science
Zitationen: 3
Dimensions
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Joseph Gottlieb Kölreuter Institut für Pflanzenwissenschaften (JKIP)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 09.2023
Sprache Englisch
Identifikator ISSN: 0028-646X, 1469-8137
KITopen-ID: 1000161319
Erschienen in New Phytologist
Verlag John Wiley and Sons
Band 239
Heft 5
Seiten 2041–2052
Vorab online veröffentlicht am 28.06.2023
Nachgewiesen in Scopus
Dimensions
Web of Science
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