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Antibiofilm assay for antimicrobial peptides combating the sulfate‐reducing bacteria Desulfovibrio vulgaris

Stillger, Lena; Viau, Lucile; Holtmann, Dirk 1; Müller, Daniela
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)


In medical, environmental, and industrial processes, the accumulation of bacteria in biofilms can disrupt many processes. Antimicrobial peptides (AMPs) are receiving increasing attention in the development of new substances to avoid or reduce biofilm formation. There is a lack of parallel testing of the effect against biofilms in this area, as well as in the testing of other antibiofilm agents. In this paper, a high-throughput screening was developed for the analysis of the antibiofilm activity of AMPs, differentiated into inhibition and removal of a biofilm. The sulfate-reducing bacterium Desulfovibrio vulgaris was used as a model organism. D. vulgaris represents an undesirable bacterium, which is considered one of the major triggers of microbiologically influenced corrosion. The application of a 96-well plate and steel rivets as a growth surface realizes real-life conditions and at the same time establishes a flexible, simple, fast, and cost-effective assay. All peptides tested in this study demonstrated antibiofilm activity, although these peptides should be individually selected depending on the addressed aim. For biofilm inhibition, the peptide DASamP1 is the most suitable, with a sustained effect for up to 21 days. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/1000161739
Veröffentlicht am 31.08.2023
DOI: 10.1002/mbo3.1376
Zitationen: 2
Web of Science
Zitationen: 2
Zitationen: 2
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 08.2023
Sprache Englisch
Identifikator ISSN: 2045-8827
KITopen-ID: 1000161739
Erschienen in MicrobiologyOpen
Verlag Wiley Open Access
Band 12
Heft 4
Seiten Art.-Nr.: e1376
Vorab online veröffentlicht am 21.08.2023
Schlagwörter antimicrobial peptides, biocorrosion, biofilm, biofilm monitoring, Desulfovibrio vulgaris
Nachgewiesen in Web of Science
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