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Biological signals support biotechnology -increasing protein yield by triggering cell death in Chlorella vulgaris through algae extract

Gusbeth, Christian 1; Müller, Alexander 1; Müller, Georg 1; Frey, Wolfgang 1
1 Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

In our previous work, we found that Chlorella vu/garis extract obtained by physical cell disruption contains a factor that causes viable cultures to die within 24 h. This so-called death factor is synthesised by C. vulgaris mainly during the stationary growth phase and is more likely to be a protein or a set of proteins. Since the sensitivity of the recipient cells and the synthesis of this factor by the donor cells depend on the stage of the cell cycle, it was concluded that the cell death is triggered by a biological signalling process. So far, it is not known exactly which molecules are involved in the signalling process that triggers cell death. However, this effect can be used to reduce the specific treatment energy during pulsed electric field (PEF) treatment. This is because, as soon as some algal cells are inactivated by the PEF treatment and the death factor is released, intact cells are also dying, leading to an increased release of intracellular components. As a result, this low-energy PEF treatment with subsequent incubation time can be used as an energy-efficient method for extracting soluble proteins and lipids in a cascade process. ... mehr

Zugehörige Institution(en) am KIT Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Publikationstyp Vortrag
Publikationsmonat/-jahr 09.2023
Sprache Englisch
Identifikator KITopen-ID: 1000162106
HGF-Programm 38.05.01 (POF IV, LK 01) Anthropogenic Carbon Cycle
Veranstaltung 16. International Bioelectrics Symposium (BIOELECTRICS 2023), Lissabon, Portugal, 10.09.2023 – 13.09.2023
Schlagwörter Chlorella vulgaris, Pulsed electric field treatment, Protein extract, Programmed cell death, Death factor
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