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Estrogenic regulation of claudin 5 and tight junction protein 1 gene expression in zebrafish: A role on blood-brain barrier?

Pellegrini, E. ; Fernezelian, D.; Malleret, C.; Gueguen, M. M.; Patche-Firmin, J.; Rastegar, S. ORCID iD icon 1; Meilhac, O.; Diotel, N.
1 Institut für Biologische und Chemische Systeme (IBCS), Karlsruher Institut für Technologie (KIT)

Abstract:

The blood-brain barrier (BBB) is a physical interface between the blood and the brain parenchyma, playing key roles in brain homeostasis. In mammals, the BBB is established thanks to tight junctions between cerebral endothelial cells, involving claudin, occludin, and zonula occludens proteins. Estrogens have been documented to modulate BBB permeability. Interestingly, in the brain of zebrafish, the estrogen-synthesizing activity is strong due to the high expression of Aromatase B protein, encoded by the cyp19a1b gene, in radial glial cells (neural stem cells). Given the roles of estrogens in BBB function, we investigated their impact on the expression of genes involved in BBB tight junctions. We treated zebrafish embryos and adult males with 17β-estradiol and observed an increased cerebral expression of tight junction and claudin 5 genes in adult males only. In females, treatment with the nuclear estrogen receptor antagonist (ICI182,780 ) had no impact. Interestingly, telencephalic injuries performed in males decreased tight junction gene expression that was partially reversed with 17β-estradiol. This was further confirmed by extravasation experiments of Evans blue showing that estrogenic treatment limits BBB leakage. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000162993
Veröffentlicht am 12.10.2023
Originalveröffentlichung
DOI: 10.1002/cne.25543
Scopus
Zitationen: 3
Web of Science
Zitationen: 3
Dimensions
Zitationen: 4
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 12.2023
Sprache Englisch
Identifikator ISSN: 0021-9967, 0092-7015, 1096-9861, 1550-7149
KITopen-ID: 1000162993
HGF-Programm 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in The journal of comparative neurology
Verlag Wiley-Liss
Band 531
Heft 17
Seiten 1828-1845
Vorab online veröffentlicht am 09.10.2023
Schlagwörter blood-brain barrier; claudin; estradiol; injury; regeneration zebrafish; tight junction protein.
Nachgewiesen in Dimensions
Web of Science
Scopus
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