Wavelength-converted light sources in fluorescence-based methods in medical technology

This contribution proposes phosphors as excitation for fluorescence analysis and evaluates their potential in this application area. Aim of this research is to provide a method which allows to quantify how well a phosphor fits as excitation in a given optical system with spectral filters for fluorescence analysis. The approach consists of a mathematical calculation of crosstalk which is first applied to abstract and subsequentially defined phosphor emission spectra. The resulting crosstalk is used as measure indicating the fit of a phosphor spectrum. Result of this contribution is a detailed description of the applied method as well as an example exercise on a given optical system which gives an impression of possibilities phosphors offer in this application. The presented method is applicable to any (new) phosphor or even LED spectra. Especially evaluations on the example optical system allow conclusions which help to design future optical systems.