O2-sensitive Mikrokavitätenarrays: 3D-Zellkultursystem mit Sensorfunktion
Gottwald, Eric
1; Grün, Christoph
1; Liebsch, Gregor
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)
1; Grün, Christoph
1; Liebsch, Gregor1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)
Abstract (englisch):
Oxygen is a crucial parameter for organotypic in vitro-cultures which
are still performed under ambient atmosphere supplemented with 5 %
CO2. This can lead to hyperoxia with its associated effects on signaling
cascades and their effects on cellular behavior. Here, we describe a
platform that enables real-time, label-free, optical oxygen (gradient)
measurements in organotypic 3D-/organoid cultures thus allowing for
physioxic culture and assay conditions in e. g. mito stress tests and
substance testing.
are still performed under ambient atmosphere supplemented with 5 %
CO2. This can lead to hyperoxia with its associated effects on signaling
cascades and their effects on cellular behavior. Here, we describe a
platform that enables real-time, label-free, optical oxygen (gradient)
measurements in organotypic 3D-/organoid cultures thus allowing for
physioxic culture and assay conditions in e. g. mito stress tests and
substance testing.
| Zugehörige Institution(en) am KIT | Institut für Funktionelle Grenzflächen (IFG) |
| Publikationstyp | Zeitschriftenaufsatz |
| Publikationsdatum | 22.11.2023 |
| Sprache | Deutsch |
| Identifikator | ISSN: 0947-0867, 1868-6249 KITopen-ID: 1000164718 |
| HGF-Programm | 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems |
| Erschienen in | Biospektrum |
| Verlag | Springer |
| Band | 29 |
| Heft | 7 |
| Seiten | 758-761 |
| Schlagwörter | 3D-Zellkultur, Sensorarrays, optische Sauerstoffmessung |
| Nachgewiesen in | OpenAlex Dimensions Scopus |
| Globale Ziele für nachhaltige Entwicklung |