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Reversible Host–Guest Crosslinks in Supramolecular Hydrogels for On‐Demand Mechanical Stimulation of Human Mesenchymal Stem Cells

Linke, Philipp; Munding, Natalie; Kimmle, Esther; Kaufmann, Stefan; Hayashi, Kentaro; Nakahata, Masaki; Takashima, Yoshinori; Sano, Masaki; Bastmeyer, Martin 1,2; Holstein, Thomas; Dietrich, Sascha; Müller-Tidow, Carsten; Harada, Akira; Ho, Anthony D.; Tanaka, Motomu
1 Zoologisches Institut (ZOO), Karlsruher Institut für Technologie (KIT)
2 Institut für Biologische und Chemische Systeme (IBCS), Karlsruher Institut für Technologie (KIT)

Abstract:

Stem cells are regulated not only by biochemical signals but also by biophysical properties of extracellular matrix (ECM). The ECM is constantly monitored and remodeled because the fate of stem cells can be misdirected when the mechanical interaction between cells and ECM is imbalanced. A well-defined ECM model for bone marrow-derived human mesenchymal stem cells (hMSCs) based on supramolecular hydrogels containing reversible host–guest crosslinks is fabricated. The stiffness (Young's modulus E) of the hydrogels can be switched reversibly by altering the concentration of non-cytotoxic, free guest molecules dissolved in the culture medium. Fine-adjustment of substrate stiffness enables the authors to determine the critical stiffness level E* at which hMSCs turn the mechano-sensory machinery on or off. Next, the substrate stiffness across E* is switched and the dynamic adaptation characteristics such as morphology, traction force, and YAP/TAZ signaling of hMSCs are monitored. These data demonstrate the instantaneous switching of traction force, which is followed by YAP/TAZ signaling and morphological adaptation. Periodical switching of the substrate stiffness across E* proves that frequent applications of mechanical stimuli drastically suppress hMSC proliferation. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000167356
Veröffentlicht am 19.01.2024
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Biologische und Chemische Systeme (IBCS)
Zoologisches Institut (ZOO)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2024
Sprache Englisch
Identifikator ISSN: 2192-2640, 2192-2659
KITopen-ID: 1000167356
HGF-Programm 47.14.02 (POF IV, LK 01) Information Storage and Processing in the Cell Nucleus
Erschienen in Advanced Healthcare Materials
Verlag Wiley-VCH Verlag
Band 13
Heft 10
Seiten Art.-Nr.: 2302607
Vorab online veröffentlicht am 20.12.2023
Nachgewiesen in Web of Science
Dimensions
Scopus
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