Besser und günstiger: Volumenreduktion in der Einzelzellgenomik

Single cell genomics (SCG) is a is a complementary method to metagenomics for exploring the genomes of uncultivated microorganisms. However, due to the minute amounts of DNA in the individual microbial cell, an amplification step is required before sequencing. Unfortunately, this reaction is notoriously costly and does not amplify all genomic regions equally well, preventing high-throughput applications and leading to incomplete and biased genomes. Here, we show a simple volume reduction approach to make SCG more feasible.