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Chromatography based profiling of feruloylated arabinans and galactans

Junker, Florian 1; Bunzel, Mirko 2
1 Karlsruher Institut für Technologie (KIT)
2 Institut für Angewandte Biowissenschaften (IAB), Karlsruher Institut für Technologie (KIT)

Abstract:

Profiling of pectic arabinans and galactans by analysis of the released oligosaccharides after backbone cleavage provides information on the complexity of the polymer structure. In plants of the family Amaranthaceae, arabinan and galactan substitution with ferulates extends the polysaccharide complexity, changing its chemical properties. Knowledge of the ferulate environment is crucial to understand structure-function-relationships of feruloylated pectins. Here, we present an approach to separate enzymatically generated feruloylated and non-feruloylated arabino- and galactooligosaccharides, followed by deesterification and semiquantitative analysis by HPAEC-PAD using previously reported relative response factors. Application of this approach to sugar beet pectins and insoluble and soluble dietary fiber preparations of amaranth and quinoa suggests that ferulates are preferably incorporated into more complex structures, as nicely demonstrated for feruloylated galactans. Also, ferulate substitution appears to negatively affect enzymatic cleavage by using endo-enzymes. As a consequence, we were able to tentatively identify new feruloylated tri- and tetrasaccharides of galactans isolated from sugar beet pectins.


Verlagsausgabe §
DOI: 10.5445/IR/1000170028
Veröffentlicht am 18.04.2024
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 04.2024
Sprache Englisch
Identifikator ISSN: 0008-6215, 1873-426X
KITopen-ID: 1000170028
Erschienen in Carbohydrate Research
Verlag Elsevier
Band 538
Seiten Art.-Nr.: 109076
Vorab online veröffentlicht am 12.03.2024
Schlagwörter Amaranthaceae, Pectins, Arabinans, Galactans, Ferulic acid, HPAEC-PAD
Nachgewiesen in Web of Science
Scopus
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