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Caenorhabditis elegans neuropeptide NLP-27 enhances neurodegeneration and paralysis in an opioid-like manner during fungal infection

Pop, Maria 1; Klemke, Anna-Lena 2; Seidler, Lena 1; Wernet, Nicole 2; Steudel, Pietrina Loredana 1; Baust, Vanessa 1; Wohlmann, Elke 2; Fischer, Reinhard 2
1 Karlsruher Institut für Technologie (KIT)
2 Institut für Angewandte Biowissenschaften (IAB), Karlsruher Institut für Technologie (KIT)

Abstract:

he nervous system of metazoans is involved in host-pathogen interactions to control immune activation. In Caenorhabditis elegans, this includes sleep induction, mediated by neuropeptide-like proteins (NLPs), which increases the chance of survival after wounding. Here we analyzed the role of NLP-27 in the infection of C. elegans with the nematode-trapping fungus Arthrobotrys flagrans. Early responses of C. elegans were the upregulation of nlp-27, the induction of paralysis (sleep), and neurodegeneration of the mechanosensing PVD (Posterior Ventral Process D) neurons. Deletion of nlp-27 reduced neurodegeneration during fungal attack. Induction of nlp-27 was independent of the MAP kinase PMK-1, and expression of nlp-27 in the hypodermis was sufficient to induce paralysis, although NLP-27 was also upregulated in head neurons. NLP-27 contains the pentapeptide YGGYG sequence known to bind the human μ- and κ-type opioid receptors suggesting NLP-27 or peptides thereof act on opioid receptors. The opioid receptor antagonist naloxone shortened the paralysis time like overexpression of NLP-27.


Verlagsausgabe §
DOI: 10.5445/IR/1000170276
Veröffentlicht am 26.04.2024
Originalveröffentlichung
DOI: 10.1016/j.isci.2024.109484
Dimensions
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Biowissenschaften (IAB)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 19.04.2024
Sprache Englisch
Identifikator ISSN: 2589-0042
KITopen-ID: 1000170276
Erschienen in iScience
Verlag Cell Press
Band 27
Heft 4
Seiten Art.-Nr.: 109484
Vorab online veröffentlicht am 12.03.2024
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