Cell-resistant wavelength-shifting molecular beacons made of l -DNA and a clickable l -configured uridine

Wavelength-shifting molecular beacons were prepared from L -DNA. The clickable anchor for the two
dyes, Cy3 and Cy5, was 2’-O-propargyl-L-uridine and was synthesized from L-ribose. Four clickable molecular
beacons were prepared and double-modified with the azide dyes by a combination of click chemistry on a
solid support for Cy3 during DNA synthesis and postsynthetic click chemistry for Cy5 in solution. Cy3 and Cy5
successfully formed a FRET pair in the beacons, and the closed form (red fluorescence) and the open form
(green fluorescence) can be distinguished by the two-color fluorescence readout. Two molecular beacons were identified to show the greatest fluorescence contrast in temperature-dependent fluorescence measurements. The stability of the L-configured molecular beacons was demonstrated after several heating and cooling cycles as well as in the cell lysate. In comparison, D-configured molecular beacons showed a rapid decrease of fluorescence contrast in the cell lysate, which is caused by the opening of the beacons, probably due to degradation. This was confirmed in cell experiments using confocal microscopy. The L-configured molecular beacons are potential intracellular thermometers for future applications.