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Inheritance of H3K9 methylation regulates genome architecture in Drosophila early embryos

Atinbayeva, Nazerke; Valent, Iris 1; Zenk, Fides; Loeser, Eva; Rauer, Michael; Herur, Shwetha; Quarato, Piergiuseppe; Pyrowolakis, Giorgos; Gomez-Auli, Alejandro; Mittler, Gerhard; Cecere, Germano; Erhardt, Sylvia ORCID iD icon 1; Tiana, Guido; Zhan, Yinxiu; Iovino, Nicola
1 Zoologisches Institut (ZOO), Karlsruher Institut für Technologie (KIT)

Abstract:

Constitutive heterochromatin is essential for transcriptional silencing and genome integrity. The establishment of constitutive heterochromatin in early embryos and its role in early fruitfly development are unknown. Lysine 9 trimethylation of histone H3 (H3K9me3) and recruitment of its epigenetic reader, heterochromatin protein 1a (HP1a), are hallmarks of constitutive heterochromatin. Here, we show that H3K9me3 is transmitted from the maternal germline to the next generation. Maternally inherited H3K9me3, and the histone methyltransferases (HMT) depositing it, are required for the organization of constitutive heterochromatin: early embryos lacking H3K9 methylation display de-condensation of pericentromeric regions, centromere-centromere de-clustering, mitotic defects, and nuclear shape irregularities, resulting in embryo lethality. Unexpectedly, quantitative CUT&Tag and 4D microscopy measurements of HP1a coupled with biophysical modeling revealed that H3K9me2/3 is largely dispensable for HP1a recruitment. Instead, the main function of H3K9me2/3 at this developmental stage is to drive HP1a clustering and subsequent heterochromatin compaction. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000171575
Veröffentlicht am 12.06.2024
Originalveröffentlichung
DOI: 10.1038/s44318-024-00127-z
Scopus
Zitationen: 1
Web of Science
Zitationen: 1
Dimensions
Zitationen: 3
Cover der Publikation
Zugehörige Institution(en) am KIT Zoologisches Institut (ZOO)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2024
Sprache Englisch
Identifikator ISSN: 0261-4189, 1460-2075
KITopen-ID: 1000171575
Erschienen in The EMBO Journal
Verlag John Wiley and Sons
Band 43
Seiten 2685 - 2714
Vorab online veröffentlicht am 03.06.2024
Schlagwörter Constitutive Heterochromatin, Early Embryogenesis, H3K9 Methylation, Intergenerational Inheritance, HP1a Clusters
Nachgewiesen in Dimensions
Web of Science
Scopus
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