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Online monitoring of continuous aqueous two-phase flotation (ATPF) for the development of an autonomous control strategy

Lohfink, Kim Carina ORCID iD icon 1,2; Baumgärtner, Katrin; Kirsch, Michel 1,2; Rhein, Frank ORCID iD icon 2; Diehl, Moritz; Nirschl, Hermann 1,2
1 Karlsruher Institut für Technologie (KIT)
2 Institut für Mechanische Verfahrenstechnik und Mechanik (MVM), Karlsruher Institut für Technologie (KIT)

Abstract:

Aqueous two-phase flotation (ATPF) is a separation technique for isolating biological products such as enzymes from crude complex biosuspensions. The continuous operation mode of ATPF allows high throughput while maintaining a high separation efficiency. In this work, a laboratory plant for continuous ATPF with integrated online measurement technology is presented and ATPF experiments with the model enzyme phospholipase A2 are performed. Three electrical conductivity probes allow real time and spatially resolved characterization of phase mixing in the flotation tank caused by rising bubbles. UV/Vis spectroscopy is used to measure the enzyme concentration online at the top phase outlet. The equilibrium concentrations in the top and bottom phase are linearly dependent on the feed concentration, revealing a constant partition coefficient. The experimental results of this work provide the basis for the development of an autonomous feedback controller for the continuous ATPF process.


Verlagsausgabe §
DOI: 10.5445/IR/1000171714
Veröffentlicht am 20.06.2024
Originalveröffentlichung
DOI: 10.1016/j.ces.2024.120287
Scopus
Zitationen: 1
Web of Science
Zitationen: 1
Dimensions
Zitationen: 1
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mechanische Verfahrenstechnik und Mechanik (MVM)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 05.09.2024
Sprache Englisch
Identifikator ISSN: 0009-2509, 1873-4405
KITopen-ID: 1000171714
Erschienen in Chemical Engineering Science
Verlag Elsevier
Band 297
Seiten Art.-NR.: 120287
Vorab online veröffentlicht am 27.05.2024
Nachgewiesen in Dimensions
Web of Science
Scopus
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