Key role of cycloalkyne nature in alkyne-dye reagents for enhanced specificity of intracellular imaging by bioorthogonal bioconjugation
Abstract:
Conjugates of benzothiophene-fused azacyclononyne BT9N-NH$_2$ with fluorescent dyes were developed
to visualise azidoglycans intracellularly. The significance of the cycloalkyne core was demonstrated by
comparing new reagents with DBCO- and BCN-dye conjugates. To reduce non-specificity during intra-
cellular bioconjugation using SPAAC, less reactive BT9N-dye reagents are preferred over highly reactive
DBCO- and BCN-dye conjugates.
to visualise azidoglycans intracellularly. The significance of the cycloalkyne core was demonstrated by
comparing new reagents with DBCO- and BCN-dye conjugates. To reduce non-specificity during intra-
cellular bioconjugation using SPAAC, less reactive BT9N-dye reagents are preferred over highly reactive
DBCO- and BCN-dye conjugates.
| Zugehörige Institution(en) am KIT | Institut für Biologische und Chemische Systeme (IBCS) Institut für Organische Chemie (IOC) |
| Publikationstyp | Zeitschriftenaufsatz |
| Publikationsmonat/-jahr | 07.2024 |
| Sprache | Englisch |
| Identifikator | ISSN: 1477-0520, 1477-0539 KITopen-ID: 1000172800 |
| Erschienen in | Organic & Biomolecular Chemistry |
| Verlag | Royal Society of Chemistry (RSC) |
| Vorab online veröffentlicht am | 03.07.2024 |
| Nachgewiesen in | Scopus Dimensions Web of Science |