Online measurement data of continuous aqueous two-phase flotation (ATPF) for enzyme purification

The .csv files contain experimental data obtained from ATPF experiments at laboratory scale.

The flotation tank used contains 700 ml of the aqueous two-phase system (ATPS). The phase forming components were polyethylene glycol 1000 and trisodium citrate dihydrate.

Phospholipase A2 was used as the model enzyme and the model suspension was mixed with the bottom phase.

The flotation tank was conceptually divided into three compartments, each compartment containing a gassing unit and an electrical conductivity probe. The electrical conductivity probes were inserted 1 cm below the phase interface.

An UV/Vis spectrometer with flow cell was implemented at the top phase outlet to obtain the top phase concentration in real-time. The bottom phase concentration was calculated via a mass balance equation.

The file names contain the process parameters in the following order: feed concentration_gas flow rate combination (Vd_1-Vd_2-Vd_3, see also below)_residence time_volume ratio (top to bottom phase)_experiment number.

The .csv files contain the following experimental data:

• t: time in min
• R_top, R_bot: top and bottom phase recirculation (R = 1 for full recirculation, R = 0 for no recirculation)
• c_bot_3, c_top_3: enzyme concentration obtained from UV/Vis measurements in % (w/w).
• kappa_bot_1, kappa_bot_2, kappa_bot_3: Electrical conductivity measurements in all three compartments in mS/cm.
• Qd_bot, Qd_top: Volume flows of top and bottom phase in ml/min.
• Vd_1, Vd_2, Vd_3: Gas volume flows of the three gassing units in ml/min.