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Access to thermostable enzymes and their application in flow biocatalysis

Stöckle, Marius 1; Peng, Martin [Beteiligte*r] ORCID iD icon 1
1 Institut für Biologische Grenzflächen (IBG), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

The immobilization of biocatalysts in a continuous fluidic setup is one way to achieve compartmentalization and thus precise control over artificial reaction cascades for synthetic chemistry. We recently demonstrated the encapsulation of unmodified thermostable enzymes in a 3D printed, agarose-based thermoreversible hydrogel to enable multi-step sequential biotransformations.[1] To test the feasibility of the encapsulation strategy, we used a naturally thermostable alcohol dehydrogenase (ADH) as well as a ketoisovalerate decarboxylase (KIVD) from a mesophile organism as exemplary biocatalysts. KIVD was thermostabilized by different computational or evolutionary methods to increase the T50 value by up to 9°C.[1,2] After the successful proof-of-concept study, we further expanded the scope of this system by integrating phenacrylate decarboxylases (PAD) into this microfluidic system.[3] As an alternative for the hydrogel based immobilization strategy, thermostable enzymes can be covalently attached onto beads in a packed-bed reactor. In this context thermostable enzymes offer improved process stability and we selected a benzaldehyde lyase (BAL) as an example, since only one enzyme had been biochemically characterized before, which was rather instable.[4] To this end, we employed a computational prediction tool[5] for the identification of a novel thermostable benzaldehyde lyase and employed the enzyme for the continuous production of α-hydroxy-ketones. ... mehr


Zugehörige Institution(en) am KIT Institut für Biologische Grenzflächen (IBG)
Publikationstyp Poster
Publikationsjahr 2023
Sprache Englisch
Identifikator KITopen-ID: 1000174905
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Veranstaltung 16th International Symposium on Biocatalysis and Biotransformations (Biotrans 2023), LaRochelle, France, 25.06.2023 – 29.06.2023
Referent/Betreuer Rabe, Kersten
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