MOSAIC: Multiscale Origami Structures as Interfaces for Cells

Over the last two decades, DNA origami has evolved into a well-established, versatile method
for the specific arrangement of molecules in the nanometre range.
Within the framework of MOSAIC (Multiscale Origami Structures as Interfaces for cells), we
structure DNA-Origami on a surface, to form micro arrays for the analysis of biological
processes Here, we focus on the phenomenon of receptor clustering, where the specific
grouping of receptors can amplify the sensitivity of a signaling response. With the complete
control over the number and spatial arrangement of ligands bound on DNA-Origami at a lower
nanoscale, MOSAIC is a compelling way to study this process. The easy adaptability of the
origami system allows a wide range of spatial arrangements and ligand-receptor relationships
to be investigated.
For MOSAIC, we prepare a surface with an oligonucleotide pattern via polymer pen lithography
(PPL). DNA origami structures are then applied to the surface using DNA-directed
immobilisation (DDI) and decorated with epidermal growth factor (EGF) in various
arrangements. In the further course, the specific activation of EGFR in adherent MCF7 cells is
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investigated. The focus lies on different intensities of activity and how these can be influenced
by the spatial arrangement of EGF on DNA origami structures.
MOSAIC combines a variety of innovative technologies such as DNA origami, PPL and DDI to
investigate fundamental aspects of early-stage cell signalling in living cells.