KIT | KIT-Bibliothek | Impressum | Datenschutz

Highly Parallel and High-Throughput Nanoliter-Scale Liquid, Cell, and Spheroid Manipulation on Droplet Microarray

Gómez, Joaquin Eduardo Urrutia ORCID iD icon 1,2
1 Institut für Automation und angewandte Informatik (IAI), Karlsruher Institut für Technologie (KIT)
2 Institut für Biologische und Chemische Systeme (IBCS), Karlsruher Institut für Technologie (KIT)

Abstract:

The droplet microarray (DMA) platform is a powerful tool for high-throughput biological and chemical applications, enabling miniaturization and parallelization of experimental processes. Capable of holding hundreds of nanoliter droplets, it facilitates the screening and analysis of various samples, including cells, bacteria, embryos, and spheroids. Handling thousands of such small volumes in parallel is essential but presents substantial challenges. In this study we utilize the open format of the DMA for controlled and highly parallel high-throughput liquid manipulations using the sandwich technique. We demonstrate high-throughput medium replacement at nanoliter-scale, maintaining high viability of cells on DMA for up to 7 days for HeLa-CLL2 cells (adherent) and SU-DHL4 cells (suspension), and up to 14 days for HEK293 spheroids. We also demonstrate highly parallel uptake aliquots from nanoliter droplets, which we use for non-destructive cell viability assessment. Additionally, the presented method enables the controlled parallel transfer of cell spheroids between different DMAs, enabling both transfer and pooling of spheroids in seconds without their damage. ... mehr


Zugehörige Institution(en) am KIT Institut für Automation und angewandte Informatik (IAI)
Institut für Biologische und Chemische Systeme (IBCS)
Publikationstyp Forschungsdaten
Publikationsdatum 05.11.2024
Erstellungsdatum 22.10.2024 - 22.10.2024
Identifikator DOI: 10.35097/61p3v2hfwxrppafw
KITopen-ID: 1000175421
Weitere HGF-Programme 43.31.02 (POF IV, LK 01) Devices and Applications
Lizenz Creative Commons Namensnennung 4.0 International
Liesmich

Cell culture and measurement data sets

Art der Forschungsdaten Dataset
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page