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Effective removal of host cell-derived nucleic acids bound to hepatitis B core antigen virus-like particles by heparin chromatography

Valentic, Angela 1; Hubbuch, Jürgen ORCID iD icon 1
1 Institut für Bio- und Lebensmitteltechnik (BLT), Karlsruher Institut für Technologie (KIT)

Abstract:

Virus-like particles (VLPs) show considerable potential for a wide array of therapeutic applications, spanning from vaccines targeting infectious diseases to applications in cancer immunotherapy and drug delivery. In the context of hepatitis B core antigen (HBcAg) VLPs, a promising candidate for gene delivery approaches, the naturally occurring nucleic acid (NA) binding region is commonly utilized for effective binding of various types of therapeutic nucleic acids (NAther). During formation of the HBcAg VLPs, host cell-derived nucleic acids (NAhc) might be associated to the NA binding region, and are thus encapsulated into the VLPs. Following a VLP harvest, the NAhc need to be removed effectively before loading the VLP with NAther. Various techniques reported in literature for this NAhc removal, including enzymatic treatments, alkaline treatment, and lithium chloride precipitation, lack quantitative evidence of sufficient NAhc removal accompanied by a subsequent high VLP protein recovery. In this study, we present a novel heparin chromatography-based process for effective NAhc removal from HBcAg VLPs. ... mehr

Zugehörige Institution(en) am KIT Institut für Bio- und Lebensmitteltechnik (BLT)
Publikationstyp Zeitschriftenaufsatz
Publikationsdatum 03.10.2024
Sprache Englisch
Identifikator ISSN: 2296-4185
KITopen-ID: 1000176407
Erschienen in Frontiers in Bioengineering and Biotechnology
Verlag Frontiers Media SA
Band 12
Seiten 1475918
Schlagwörter virus-like particles, heparin chromatography, HBcAg, host cell-derived nucleic acids, process development
Nachgewiesen in Scopus
Web of Science
Dimensions
Globale Ziele für nachhaltige Entwicklung Ziel 3 – Gesundheit und Wohlergehen

Verlagsausgabe §
DOI: 10.5445/IR/1000176407
Veröffentlicht am 19.11.2024
Seitenaufrufe: 28
seit 19.11.2024
Downloads: 17
seit 20.11.2024
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