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Biological Signalling Supports Biotechnology: Cell Death Triggers Protein Release from Chlorella vulgaris

Gusbeth, Christian 1
1 Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM), Karlsruher Institut für Technologie (KIT)

Abstract (englisch):

Multiple studies have demonstrated that following Pulsed Electric Fields (PEF) treatment, an incubation period exceeding 6 h in buffer is necessary to enhance the bio-accessibility of cell components such as proteins and lipids. This enhancement is primarily attributed to enzymatic processes activated during incubation, which facilitate the release of proteins and promote the solvent extraction of lipids from various microalgal species. The induction of this autolytic process can be triggered by various means, including dark anoxia, where microalgae are incubated in darkness for 24 to 48 h, or even by PEF treatment with very low specific energies (< 5 J/g). Notably, in Chlorella vulgaris, there's evidence suggesting the presence of a specific cell death inducing factor (CDIF) of protein origin, which triggers cell death in intact cells. The prevailing concept suggests that CDIF plays a pivotal role during the algae incubation period post-PEF treatment, prompting cell death and subsequent autolytic processes, thereby enhancing the extraction process. The search for the identity of the CDIF led to the assumption that radical oxygen species (ROS) may play a decisive role as signalling molecules, which are involved in many cell signalling pathways. ... mehr


Zugehörige Institution(en) am KIT Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Publikationstyp Vortrag
Publikationsmonat/-jahr 10.2024
Sprache Englisch
Identifikator KITopen-ID: 1000177251
HGF-Programm 38.05.01 (POF IV, LK 01) Anthropogenic Carbon Cycle
Veranstaltung 4th Baltic Biophysics Conference (2024), Kaunas, Litauen, 01.10.2024 – 03.10.2024
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