Accelerating NMR spectroscopy via parallel SABRE in a continuous-flow system

Nuclear Magnetic Resonance (NMR) techniques are inherently limited by low thermal polarization, often requiring time-consuming signal averaging to achieve sufficient sensitivity. Parallel detection using multiple decoupled coils improves sample throughput by enabling simultaneous acquisition from multiple samples, partially mitigating these limitations. To further enhance detection sensitivity and acquisition efficiency, we investigated the integration of Signal Amplification By Reversible Exchange (SABRE) hyperpolarization with parallel NMR spectroscopy detection. SABRE significantly increases nuclear spin polarization, allowing high-SNR signal acquisition within a single scan. In this study, hyperpolarized solutions were continuously generated using tube-in-tube reactors and delivered to dual detection coils operated in parallel within a 1.05 T MRI system, enabling simultaneous acquisition of hyperpolarized signals under continuous-flow conditions. Complementary MRI experiments directly demonstrated the sensitivity enhancement achievable with SABRE hyperpolarization, particularly for low-concentration analytes. Building on this, parallel SABRE experiments further validated the feasibility of combining hyperpolarization with simultaneous multi-channel NMR detection to improve measurement efficiency without requiring signal averaging. ... mehrThis work provides an experimental basis for high-throughput NMR detection of low-concentration liquid samples under continuous-flow conditions, with promising potential for applications in metabolic studies and pharmaceutical screening.