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Particulate matter from both heavy fuel oil and diesel fuel shipping emissions show strong biological effects on human lung cells at realistic and comparable in vitro exposure conditions

Oeder, S.; Kanashova, T.; Sippula, O.; Sapcariu, S. C.; Streibel, T.; Arteaga-Salas, J. M.; Passig, J.; Dilger, M. 1,2; Paur, H. R. 2; Schlager, C. 2; Mülhopt, S. ORCID iD icon 2; Diabate, S. 1; Weiss, C. 1; Stengel, B.; Rabe, R.; Harndorf, H.; Torvela, T.; Jokiniemi, J. K.; Hirvonen, M. R.; ... mehr


Background: Ship engine emissions are important with regard to lung and cardiovascular diseases especially in coastal regions worldwide. Known cellular responses to combustion particles include oxidative stress and inflammatory signalling.
Objectives: To provide a molecular link between the chemical and physical characteristics of ship emission particles and the cellular responses they elicit and to identify potentially harmful fractions in shipping emission aerosols.
Methods: Through an air-liquid interface exposure system, we exposed human lung cells under realistic in vitro conditions to exhaust fumes from a ship engine running on either common heavy fuel oil (HFO) or cleaner-burning diesel fuel (DF). Advanced chemical analyses of the exhaust aerosols were combined with transcriptional, proteomic and metabolomic profiling including isotope labelling methods to characterise the lung cell responses.
Results: The HFO emissions contained high concentrations of toxic compounds such as metals and polycyclic aromatic hydrocarbon, and were higher in particle mass. These compounds were lower in DF emissions, which in turn had higher concentrations of elemental carbon (“soot”). ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/110101331
Veröffentlicht am 17.01.2018
DOI: 10.1371/journal.pone.0126536
Zitationen: 106
Web of Science
Zitationen: 101
Zitationen: 116
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Technische Chemie (ITC)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 1932-6203
KITopen-ID: 110101331
HGF-Programm 47.01.01 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. ITG+ITC
Erschienen in PLoS one
Verlag Public Library of Science (PLoS)
Band 10
Heft 6
Seiten e126536
Nachgewiesen in Dimensions
Web of Science
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