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Particulate matter from both heavy fuel oil and diesel fuel shipping emissions show strong biological effects on human lung cells at realistic and comparable in vitro exposure conditions

Oeder, S.; Kanashova, T.; Sippula, O.; Sapcariu, S.C.; Streibel, T.; Arteaga-Salas, J.M.; Passig, J.; Dilger, M.; Paur, H.R.; Schlager, C.; Mülhopt, S.; Diabate, S.; Weiss, C.; Stengel, B.; Rabe, R.; Harndorf, H.; Torvela, T.; Jokiniemi, J.K.; Hirvonen, M.R.; Schmidt-Weber, C.; ... mehr

Abstract:
Background: Ship engine emissions are important with regard to lung and cardiovascular diseases especially in coastal regions worldwide. Known cellular responses to combustion particles include oxidative stress and inflammatory signalling.
Objectives: To provide a molecular link between the chemical and physical characteristics of ship emission particles and the cellular responses they elicit and to identify potentially harmful fractions in shipping emission aerosols.
Methods: Through an air-liquid interface exposure system, we exposed human lung cells under realistic in vitro conditions to exhaust fumes from a ship engine running on either common heavy fuel oil (HFO) or cleaner-burning diesel fuel (DF). Advanced chemical analyses of the exhaust aerosols were combined with transcriptional, proteomic and metabolomic profiling including isotope labelling methods to characterise the lung cell responses.
Results: The HFO emissions contained high concentrations of toxic compounds such as metals and polycyclic aromatic hydrocarbon, and were higher in particle mass. These compounds were lower in DF emissions, which in turn had higher co ... mehr


Zugehörige Institution(en) am KIT Institut für Technische Chemie (ITC)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 1932-6203
URN: urn:nbn:de:swb:90-AAA1101013314
KITopen ID: 110101331
HGF-Programm 47.01.01; LK 01
Erschienen in PLoS one
Band 10
Heft 6
Seiten e126536
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