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Verlagsausgabe
DOI: 10.5445/IR/110101622
Veröffentlicht am 17.01.2018
Originalveröffentlichung
DOI: 10.1371/journal.ppat.1004663
Scopus
Zitationen: 54
Web of Science
Zitationen: 49

CD44 Plays a Functional Role in Helicobacter pylori-induced Epithelial Cell Proliferation

Bertaux-Skeirik, Nina; Feng, Rui; Schumacher, Michael A.; Li, Jing; Mahe, Maxime M.; Engevik, Amy C.; Javier, Jose E.; Peek, Richard M.; Ottemann, Karen; Orian-Rousseau, Veronique; Boivin, Gregory P.; Helmrath, Michael A.; Zavros, Yana

Abstract:
The cytotoxin-associated gene (Cag) pathogenicity island is a strain-specific constituent of Helicobacter pylori (H. pylori) that augments cancer risk. CagA translocates into the cytoplasm where it stimulates cell signaling through the interaction with tyrosine kinase c-Met receptor, leading cellular proliferation. Identified as a potential gastric stem cell marker, cluster-of-differentiation (CD) CD44 also acts as a co-receptor for c-Met, but whether it plays a functional role in H. pylori-induced epithelial proliferation is unknown. We tested the hypothesis that CD44 plays a functional role in H. pylori-induced epithelial cell proliferation. To assay changes in gastric epithelial cell proliferation in relation to the direct interaction with H. pylori, human- and mouse-derived gastric organoids were infected with the G27 H. pylori strain or a mutant G27 strain bearing cagA deletion (ΔCagA::cat). Epithelial proliferation was quantified by EdU immunostaining. Phosphorylation of c-Met was analyzed by immunoprecipitation followed by Western blot analysis for expression of CD44 and CagA. H. pylori infection of both mouse- and human-deri ... mehr


Zugehörige Institution(en) am KIT Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 1553-7366, 1553-7374
URN: urn:nbn:de:swb:90-AAA1101016221
KITopen ID: 110101622
HGF-Programm 47.01.01; LK 01
Erschienen in PLoS pathogens
Band 11
Heft 2
Seiten 1-24/e1004663
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