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DOI: 10.5445/IR/110103704

Quantitative DNA Analyses for Airborne Birch Pollen

Müller-Germann, Isabell; Vogel, Bernhard; Vogel, Heike; Pauling, Andreas; Fröhlich-Nowolsky, Janine; Pöschl, Ulrich; Despres, Viviane R.

Abstract:
Birch trees produce large amounts of highly allergenic pollen grains that are distributed by wind and impact human health by causing seasonal hay fever, pollen-related asthma, and other allergic diseases. Traditionally, pollen forecasts are based on conventional microscopic counting techniques that are labor-intensive and limited in the reliable identification of species. Molecular biological techniques provide an alternative approach that is less labor-intensive and enables identification of any species by its genetic fingerprint. A particularly promising method is quantitative Real-Time polymerase chain reaction (qPCR), which can be used to determine the number of DNA copies and thus pollen grains in air filter samples. During the birch pollination season in 2010 in Mainz, Germany, we collected air filter samples of fine (<3 μm) and coarse air particulate matter. These were analyzed by qPCR using two different primer pairs: one for a single-copy gene (BP8) and the other for a multi-copy gene (ITS). The BP8 gene was better suitable for reliable qPCR results, and the qPCR results obtained for coarse particulate matter were well c ... mehr


Zugehörige Institution(en) am KIT Institut für Meteorologie und Klimaforschung - Forschungsbereich Troposphäre (IMK-TRO)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator ISSN: 1932-6203
URN: urn:nbn:de:swb:90-AAA1101037045
KITopen ID: 110103704
HGF-Programm 12.01.01; LK 01
Erschienen in PLoS one
Band 10
Heft 10
Seiten Art.Nr. e0140949
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