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Quantitative DNA Analyses for Airborne Birch Pollen

Müller-Germann, Isabell; Vogel, Bernhard 1; Vogel, Heike ORCID iD icon 1; Pauling, Andreas; Fröhlich-Nowolsky, Janine; Pöschl, Ulrich; Despres, Viviane R.
1 Institut für Meteorologie und Klimaforschung (IMK), Karlsruher Institut für Technologie (KIT)

Abstract:

Birch trees produce large amounts of highly allergenic pollen grains that are distributed by wind and impact human health by causing seasonal hay fever, pollen-related asthma, and other allergic diseases. Traditionally, pollen forecasts are based on conventional microscopic counting techniques that are labor-intensive and limited in the reliable identification of species. Molecular biological techniques provide an alternative approach that is less labor-intensive and enables identification of any species by its genetic fingerprint. A particularly promising method is quantitative Real-Time polymerase chain reaction (qPCR), which can be used to determine the number of DNA copies and thus pollen grains in air filter samples. During the birch pollination season in 2010 in Mainz, Germany, we collected air filter samples of fine (<3 μm) and coarse air particulate matter. These were analyzed by qPCR using two different primer pairs: one for a single-copy gene (BP8) and the other for a multi-copy gene (ITS). The BP8 gene was better suitable for reliable qPCR results, and the qPCR results obtained for coarse particulate matter were well correlated with the birch pollen forecasting results of the regional air quality model COSMO-ART. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/110103704
Veröffentlicht am 17.01.2018
Originalveröffentlichung
DOI: 10.1371/journal.pone.0140949
Scopus
Zitationen: 18
Web of Science
Zitationen: 14
Dimensions
Zitationen: 20
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Meteorologie und Klimaforschung Troposphärenforschung (IMKTRO)
KIT-Zentrum Klima und Umwelt (ZKU)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 1932-6203
urn:nbn:de:swb:90-AAA1101037045
KITopen-ID: 110103704
HGF-Programm 12.01.01 (POF III, LK 01) Clouds in a pertubed atmosphere
Erschienen in PLoS one
Verlag Public Library of Science (PLoS)
Band 10
Heft 10
Seiten Art.Nr. e0140949
Nachgewiesen in Scopus
Dimensions
Web of Science
Globale Ziele für nachhaltige Entwicklung Ziel 3 – Gesundheit und Wohlergehen
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