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Hematopoietic and mesenchymal stem cells: Polymeric nanoparticle uptake and lineage differentiation

Brüstle, I.; Simmet, T.; Nienhaus, G. U. ORCID iD icon 1,2; Landfester, K.; Mailänder, V.
1 Institut für Angewandte Physik (APH), Karlsruher Institut für Technologie (KIT)
2 Institut für Toxikologie und Genetik (ITG), Karlsruher Institut für Technologie (KIT)


The combination of stem cell therapy and nanoparticles promises to enhance the effect of cellular therapies by using nanocarriers as drug delivery devices to guide the further differentiation or homing of stem cells. The impact of nanoparticles on primary cell types remains much more elusive as most groups study the nanoparticle–cell interaction in malignant cell lines. Here, we report on the influence of polymeric nanoparticles on human hematopoietic stem cells (hHSCs) and mesenchymal stem cells (hMSCs). In this study we systematically investigated the influence of polymeric nanoparticles on the cell functionality and differentiation capacity of hHSCs and hMSCs to obtain a deeper knowledge of the interaction of stem cells and nanoparticles. As model systems of nanoparticles, two sets of either bioinert (polystyrene without carboxylic groups on the surface) or biodegradable (PLLA without magnetite) particles were analyzed. Flow cytometry and microscopy analysis showed high uptake rates and no toxicity for all four tested particles in hMSCs and hHSCs. During the differentiation process, the payload of particles per cell decreased. The PLLA–Fe particle showed a significant increase in the IL-8 release in hMSCs but not in hHSCs. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/110103824
DOI: 10.3762/bjnano.6.38
Zitationen: 17
Zitationen: 19
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Institut für Nanotechnologie (INT)
Institut für Toxikologie und Genetik (ITG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 2190-4286
KITopen-ID: 110103824
HGF-Programm 43.23.01 (POF III, LK 01) Advanced Optical Lithography+Microscopy
Erschienen in Beilstein journal of nanotechnology
Verlag Beilstein-Institut
Band 6
Heft 1
Seiten 383-395
Schlagwörter cytokine secretion, differentiation, hematopoietic stem cells, mesenchymal stem cells, nanoparticles
Nachgewiesen in Web of Science
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