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Glycolipids produced by Rouxiella sp. DSM 100043 and isolation of the biosurfactants via foam-fractionation

Kügler, J.H.; Muhle-Goll, C.; Hansen, S.H.; Völp, A.R.; Kirschhöfer, F.; Kühl, B.; Brenner-Weiss, G.; Luy, B.; Syldatk, C.; Hausmann, R.

Abstract:
Microorganisms produce a great variety of secondary metabolites that feature surface active and bioactive properties. Those possessing an amphiphilc molecular structure are also termed biosurfactant and are of great interest due to their often unique properties. Rouxiella sp. DSM 100043 is a gram negative enterobacter isolated from peat-bog soil and described as a new biosurfactant producing species in this study. Rouxiella sp. produces glycolipids, biosurfactants with a carbohydrate moiety in its structure. This study characterizes the composition of glycolipids with different hydrophobicities that have been produced during cultivation in a bioreactor and been extracted and purified from separated foam. Using two dimensional nuclear magnetic resonance spectroscopy, the hydrophilic moieties are elucidated as glucose with various acylation sites and as talose within the most polar glycolipids. The presence of 3′ hydroxy lauroleic acid as well as myristic and myristoleic acid has been detected.

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Volltext §
DOI: 10.5445/IR/110104045
Originalveröffentlichung
DOI: 10.1186/s13568-015-0167-7
Scopus
Zitationen: 6
Web of Science
Zitationen: 4
Dimensions
Zitationen: 3
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 2191-0855
urn:nbn:de:swb:90-AAA1101040452
KITopen-ID: 110104045
HGF-Programm 47.02.06 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in AMB express
Verlag SpringerOpen
Band 5
Heft 1
Seiten 82/1-11
Schlagwörter Biosurfactant, Surfactant, Emulsifier, Glycolipid, Rouxiella, Serratia, Talose, Hydroxy linoleic acid, Myristic acid, Myristoleic acid
Nachgewiesen in Scopus
Web of Science
Dimensions
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