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Survival and ice nucleation activity of bacteria as aerosols in a cloud simulation chamber

Amato, P.; Joly, M.; Schaupp, C. 1; Attard, E.; Möhler, O. 1; Morris, C. E.; Brunet, Y.; Delort, A. M.
1 Institut für Meteorologie und Klimaforschung (IMK), Karlsruher Institut für Technologie (KIT)


The residence time of bacterial cells in the atmosphere is predictable by numerical models. However, estimations of their aerial dispersion as living entities are limited by a lack of information concerning survival rates and behavior in relation to atmospheric water. Here we investigate the viability and ice nucleation (IN) activity of typical atmospheric ice nucleation active bacteria (Pseudomonas syringae and P. fluorescens) when airborne in a cloud simulation chamber (AIDA, Karlsruhe, Germany). Cell suspensions were sprayed into the chamber and aerosol samples were collected by impingement at designated times over a total duration of up to 18 h, and at some occasions after dissipation of a cloud formed by depressurization. Aerosol concentration was monitored simultaneously by online instruments. The cultivability of airborne cells decreased exponentially over time with a half-life time of 250 ±30 min (about 3.5 to 4.5 h). In contrast, IN activity remained unchanged for several hours after aerosolization, demonstrating that IN activity was maintained after cell death. Interestingly, the relative abundance of IN active cells still airborne in the chamber was strongly decreased after cloud formation and dissipation. ... mehr

Verlagsausgabe §
DOI: 10.5445/IR/110104153
Veröffentlicht am 19.10.2018
DOI: 10.5194/acp-15-6455-2015
Zitationen: 72
Web of Science
Zitationen: 69
Zitationen: 87
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Meteorologie und Klimaforschung Atmosphärische Aerosolforschung (IMKAAF)
KIT-Zentrum Klima und Umwelt (ZKU)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 1680-7316, 1680-7324
KITopen-ID: 110104153
HGF-Programm 12.01.01 (POF III, LK 01) Clouds in a pertubed atmosphere
Erschienen in Atmospheric chemistry and physics
Verlag European Geosciences Union (EGU)
Band 15
Seiten 6455-6465
Nachgewiesen in Dimensions
Web of Science
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