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The absence of the Pseudomonas aeruginosa OprF protein leads to increased biofilm formation through variation in c-di-GMP level

Bouffartigues, E.; Moscoso, J. A.; Duchesne, R.; Rosay, T.; Fito-Boncmpte, L.; Gicquel, G.; Maillot, O.; Benard, M.; Bazire, A.; Brenner-Weiss, G. 1; Lesouhaitier, O.; Lerouge, P.; Dufour, A.; Orange, N.; Feuilloley, M. G. J.; Overhage, J. 1; Filloux, A.; Chevalier, S.
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)


OprF is the major outer membrane porin in bacteria belonging to the Pseudomonas genus. In previous studies, we have shown that OprF is required for full virulence expression of the opportunistic pathogen Pseudomonas aeruginosa. Here, we describe molecular insights on the nature of this relationship and report that the absence of OprF leads to increased biofilm formation and production of the Pel exopolysaccharide. Accordingly, the level of c-di-GMP, a key second messenger in biofilm control, is elevated in an oprF mutant. By decreasing c-di-GMP levels in this mutant, both biofilm formation and pel gene expression phenotypes were restored to wild-type levels. We further investigated the impact on two small RNAs, which are associated with the biofilm lifestyle, and found that expression of rsmZ but not of rsmY was increased in the oprF mutant and this occurs in a c-di-GMP-dependent manner. Finally, the extracytoplasmic function (ECF) sigma factors AlgU and SigX displayed higher activity levels in the oprF mutant. Two genes of the SigX regulon involved in c-di-GMP metabolism, PA1181 and adcA (PA4843), were up-regulated in the oprF mutant, partly explaining the increased c-di-GMP level. ... mehr

Volltext §
DOI: 10.5445/IR/110104652
DOI: 10.3389/fmicb.2015.00630
Zitationen: 61
Web of Science
Zitationen: 58
Zitationen: 65
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2015
Sprache Englisch
Identifikator ISSN: 1664-302X
KITopen-ID: 110104652
HGF-Programm 47.02.06 (POF III, LK 01) Zellpopul.auf Biofunk.Oberflächen IFG
Erschienen in Frontiers in microbiology
Verlag Frontiers Media SA
Band 6
Seiten 630
Schlagwörter OprF, exopolysaccharides, biofilm, c-di-GMP, SigX, ECF
Nachgewiesen in Dimensions
Web of Science
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