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The absence of the Pseudomonas aeruginosa OprF protein leads to increased biofilm formation through variation in c-di-GMP level

Bouffartigues, E.; Moscoso, J.A.; Duchesne, R.; Rosay, T.; Fito-Boncmpte, L.; Gicquel, G.; Maillot, O.; Benard, M.; Bazire, A.; Brenner-Weiss, G.; Lesouhaitier, O.; Lerouge, P.; Dufour, A.; Orange, N.; Feuilloley, M.G.J.; Overhage, J.; Filloux, A.; Chevalier, S.

OprF is the major outer membrane porin in bacteria belonging to the Pseudomonas genus. In previous studies, we have shown that OprF is required for full virulence expression of the opportunistic pathogen Pseudomonas aeruginosa. Here, we describe molecular insights on the nature of this relationship and report that the absence of OprF leads to increased biofilm formation and production of the Pel exopolysaccharide. Accordingly, the level of c-di-GMP, a key second messenger in biofilm control, is elevated in an oprF mutant. By decreasing c-di-GMP levels in this mutant, both biofilm formation and pel gene expression phenotypes were restored to wild-type levels. We further investigated the impact on two small RNAs, which are associated with the biofilm lifestyle, and found that expression of rsmZ but not of rsmY was increased in the oprF mutant and this occurs in a c-di-GMP-dependent manner. Finally, the extracytoplasmic function (ECF) sigma factors AlgU and SigX displayed higher activity levels in the oprF mutant. Two genes of the SigX regulon involved in c-di-GMP metabolism, PA1181 and adcA (PA4843), were up-regulated in the oprF muta ... mehr

Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2015
Sprache Englisch
Identifikator DOI: 10.3389/fmicb.2015.00630
ISSN: 1664-302X
URN: urn:nbn:de:swb:90-AAA1101046527
KITopen ID: 110104652
HGF-Programm 47.02.06; LK 01
Erschienen in Frontiers in microbiology
Band 6
Seiten 630
Lizenz CC BY 4.0: Creative Commons Namensnennung 4.0 International
Schlagworte OprF, exopolysaccharides, biofilm, c-di-GMP, SigX, ECF
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