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Use of culture-independent techniques for food industries water surveillance

Varela Villarreal, J.; Schwartz, T.; Obst, U.; Rovira, J.

Abstract:
Drinking water that comes from public suppliers is not sterile, it contains a number of autochthonous and mostly harmless bacteria. But, pathogenic or opportunistic bacteria may enter drinking water facilities in case of irregular operating conditions. In this case, some of these bacteria are able to persist and become distributed to the food production lines. If pathogens find their optimal growth conditions (e.g., nutrient, humidity and temperature) a proliferation and transfer to humans becomes a threaten. Thus, drinking water is controlled in the framework of the EU project PathogenCombat as a possible source of contamination during food production. Our challenge within the project is to use culture-independent techniques to detect pathogenic bacteria in water of food industry and to look for possible water-derived critical control points in the production lines. Techniques such as PCR and Real Time q-PCR, are applied to specifically detect Listeria monocytogenes, Mycobacterium avium subsp. paratuberculosis, Campylobacter jejuni, Enterococcus ssp, Salmonella ssp, Escherichia coli, and Pseudomonas aeruginosa in different points o ... mehr


Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Proceedingsbeitrag
Jahr 2009
Sprache Englisch
Identifikator KITopen-ID: 120078891
HGF-Programm 47.04.02 (POF II, LK 01)
Erschienen in Jahrestagung der Vereinigung für Allgemeine und Angewandte Mikrobiologie (VAAM), Bochum, 8.-11.März 2009
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