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URN: urn:nbn:de:swb:90-AAA203220014

Kombinationswirkungen umweltrelevanter Metallverbindungen in Lungenzellen

Schwerdtle, Tanja; Pelzer, Anke; Seidel, A.; Hartwig, Andrea

Abstract:

This project aims to investigate the genotoxicity of
environmentally relevant particulate nickel and cadmium
compounds in human lung cells as primary targets of
metal-induced carcinogenicity. Special focus will be given to
the question whether direct DNA damage or inhibition of DNA
repair processes causing enhancing effects with other DNA
damaging agents are predominant. In a first step, a sensitive
procedure to quantify DNA adducts of benzo[a]pyrene (B[a]P) was
established. This method is based on HPLC with fluorescence
detection and allows the specific and reproducible
quantification of 1 DNA-adduct of benzo[a]pyrene diol epoxide
(BPDE) per 108 base pairs in cultured cells. By this approach,
we demonstrated the dose-dependent generation of DNA lesions
and their repair in HeLa and A549 lung cells. The removal of DNA
adducts of BPDE was inhibited by both NiCl2 and NiO in the
non-cytotoxic concentration range. The results obtained so far
indicate that the repair inhibition by Ni(II) observed
previously also applies for lung cells and for both soluble and
insoluble nickel compounds.

Zusammenfassung

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Zugehörige Institution(en) am KIT Institut für Lebensmittelchemie und Toxikologie (ILMCT)
Publikationstyp Buchaufsatz
Jahr 2001
Sprache Englisch
Identifikator URN: urn:nbn:de:swb:90-AAA203220014
KITopen ID: 20322001
Erscheinungsvermerk In: Diskussionskreise beim Statusseminar des BWPLUS 2001 [online]. Zsgest. v. F. Horsch. Karlsruhe 2001. (FZKA-BWPLUS. 75.)
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