Synthese und Anwendung funktioneller magnetischer Mikro- und Nanopartikel zur Aufreinigung von Proteinen
Abstract:
Work focused on the development of a low-cost process to synthesize functional magnetic micro- and nanoparticles and the demonstration of their use for bioseparation. Magnetic particles were produced by silane coating of mineral nanoparticles as well as by means of mini emulsion or suspension polymerization processes. Particles were functionalized with dye ligands and strongly and weakly acid cation exchanger groups and they are used for the purification of model enzymes from biosuspension.
| Zugehörige Institution(en) am KIT | Institut für Funktionelle Grenzflächen (IFG) |
| Publikationstyp | Hochschulschrift |
| Publikationsjahr | 2009 |
| Sprache | Deutsch |
| Identifikator | urn:nbn:de:swb:90-108052 KITopen-ID: 1000010805 |
| HGF-Programm | 47.01.04 (POF II, LK 01) Biologische Schlüsselmoleküle IFG |
| Verlag | Universität Karlsruhe (TH) |
| Umfang | IX, 174 S. |
| Art der Arbeit | Dissertation |
| Fakultät | Fakultät für Chemieingenieurwesen und Verfahrenstechnik (CIW) |
| Institut | Institut für Funktionelle Grenzflächen (IFG) |
| Prüfungsdaten | 27.01.2009 |
| Prüfungsdatum | 27.01.2009 |
| Schlagwörter | Bio-saparation; Downstream processing; enzyme purification; Magnetic separation; micro- nanoparticle; particle functionalization; Affinity Ligand |
| Referent/Betreuer | Franzreb, M. |