Development of High Throughput Screening Methods for the Automated Optimization of Inclusion Body Protein Refolding Processes

In this study robotic HTS methods for protein refolding were developed with a focus on HTS compatible analytics. Solubility was identified as a meaningful objective function during refolding processes. Measurement of protein solubility in a highly complex buffer matrix with varying protein conformations was established. A genetic algorithm in combination with DoE was used for an effective and fully automated optimization of 5 process parameters in parallel in a wide parameters space.