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Ultra-fast, high-precision Image Analysis for Localization-based Superresolution Microscopy

Quan, Tingwei; Li, Pengcheng; Long, Fan; Zeng, Shaoqun; Luo, Qingming; Hedde, Per Niklas; Nienhaus, Gerd Ulrich; Huang, Zhen-Li

Localization-based super resolution microscopy holds superior performances in live cell imaging, but its widespread use is thus far mainly hindered by the slow image analysis speed. Here we show a powerful image analysis method based on the combination of the maximum likelihood algorithm and a Graphics Processing Unit (GPU). Results indicate that our method is fast enough for real-time processing of experimental images even from fast EMCCD cameras working at full frame rate without compromising localization precision or field of view. This newly developed method is also capable of revealing movements from the images immediately after data acquisition, which is of great benefit to live cell imaging.

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DOI: 10.1364/OE.18.011867
Zitationen: 56
Web of Science
Zitationen: 46
Zugehörige Institution(en) am KIT Institut für Angewandte Physik (APH)
Publikationstyp Zeitschriftenaufsatz
Jahr 2010
Sprache Englisch
Identifikator ISSN: 1094-4087
KITopen-ID: 1000027198
Erschienen in Optics express
Band 18
Heft 11
Seiten 11867-11876
Nachgewiesen in Web of Science
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