Development of a methodology linking protein phase behavior and Hydrophobic Interaction Chromatography

This thesis deals with the development of a universal methodology for the generation of protein phase diagrams in high-throughput in microbatch scale. The main influencing factors on binding behavior using Hydrophobic Interaction Chromatography (HIC) have been determined and new approaches for increasing binding capacity of HIC adsorbers have been developed. Additionally, fast tools have been investigated to predict binding behavior in HIC and to estimate protein phase behavior.