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URN: urn:nbn:de:swb:90-722119

A genotypic analysis of five P. aeruginosa strains after biofilm infection by phages targeting different cell surface receptors

Pires, D. P.; Dötsch, A.; Anderson, E. M.; Hao, Y.; Khursigara, C. M.; Lam, J. S.; Sillankorva, S.; Azeredo, J.

Abstract:
Antibiotic resistance constitutes one of the most serious threats to the global public health and urgently requires new and effective solutions. Bacteriophages are bacterial viruses increasingly recognized as being good alternatives to traditional antibiotic therapies. In this study, the efficacy of phages, targeting different cell receptors, against
Pseudomonas aeruginosa PAO1 biofilm and planktonic cell cultures was evaluated over the course of 48 h. Although significant reductions in the number of viable cells were achieved for both cases, the high level of adaptability of the bacteria in response to the selective pressure caused by phage treatment resulted in the emergence of phageresistant variants. To further investigate the genetic makeup of phage-resistant variants isolated from biofilm infection experiments, some of these bacteria were selected for phenotypic and genotypic characterization. Whole genome sequencing was performed on five phage-resistant variants and all of them carried mutations affecting the galU gene as well as one of pil genes. The sequencing analysis further revealed that three of the P. aeruginosa PAO1 ... mehr


Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Jahr 2017
Sprache Englisch
Identifikator ISSN: 1664-302X
URN: urn:nbn:de:swb:90-722119
KITopen ID: 1000072211
HGF-Programm 47.02.06; LK 01
Erschienen in Frontiers in microbiology
Band 8
Seiten Art. Nr. 1229
Schlagworte biofilms, bacteriophages, P. aeruginosa, bacterial resistance
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