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3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study

Shkarina, Svetlana 1; Shkarin, Roman 2; Weinhardt, Venera 1,2; Melnik, Elizaveta; Vacun, Gabriele; Kluger, Petra; Loza, Kateryna; Epple, Matthias; Ivlev, Sergei I.; Baumbach, Tilo 1,2; Surmeneva, Maria A.; Surmenev, Roman A.
1 Institut für Photonenforschung und Synchrotronstrahlung (IPS), Karlsruher Institut für Technologie (KIT)
2 Laboratorium für Applikationen der Synchrotronstrahlung (LAS), Karlsruher Institut für Technologie (KIT)


To date, special interest has been paid to composite scaffolds based on polymers enriched with hydroxyapatite (HA). However, the role of HA containing different trace elements such as silicate in the structure of a polymer scaffold has not yet been fully explored. Here, we report the potential use of silicate-containing hydroxyapatite (SiHA) microparticles and microparticle aggregates in the predominant range from 2.23 to 12.40 µm in combination with polycaprolactone (PCL) as a hybrid scaffold with randomly oriented and well-aligned microfibers for regeneration of bone tissue. Chemical and mechanical properties of the developed 3D scaffolds were investigated with XRD, FTIR, EDX and tensile testing. Furthermore, the internal structure and surface morphology of the scaffolds were analyzed using synchrotron X-ray µCT and SEM. Upon culturing human mesenchymal stem cells (hMSC) on PCL-SiHA scaffolds, we found that both SiHA inclusion and microfiber orientation affected cell adhesion. The best hMSCs viability was revealed at 10 day for the PCL-SiHA scaffolds with well-aligned structure (~82%). It is expected that novel hybrid scaffolds of PCL will improve tissue ingrowth in vivo due to hydrophilic SiHA microparticles in combination with randomly oriented and well-aligned PCL microfibers, which mimic the structure of extracellular matrix of bone tissue.

Verlagsausgabe §
DOI: 10.5445/IR/1000083767
Veröffentlicht am 22.06.2018
DOI: 10.1038/s41598-018-27097-7
Zitationen: 109
Web of Science
Zitationen: 105
Zitationen: 115
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Informatik (IAI)
Institut für Angewandte Informatik und Formale Beschreibungsverfahren (AIFB)
Institut für Industrielle Informationstechnik (IIIT)
Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2018
Sprache Englisch
Identifikator ISSN: 2045-2322
KITopen-ID: 1000083767
HGF-Programm 56.03.30 (POF III, LK 01) Soft Matter, Health and Life Sciences
Erschienen in Scientific reports
Verlag Nature Research
Band 8
Heft 1
Seiten Article: 8907
Vorab online veröffentlicht am 11.06.2018
Nachgewiesen in Dimensions
Web of Science
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