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DOI: 10.5445/IR/1000083767
Veröffentlicht am 22.06.2018
DOI: 10.1038/s41598-018-27097-7

3D biodegradable scaffolds of polycaprolactone with silicate-containing hydroxyapatite microparticles for bone tissue engineering: high-resolution tomography and in vitro study

Shkarina, Svetlana; Shkarin, Roman; Weinhardt, Venera; Melnik, Elizaveta; Vacun, Gabriele; Kluger, Petra; Loza, Kateryna; Epple, Matthias; Ivlev, Sergei I.; Baumbach, Tilo; Surmeneva, Maria A.; Surmenev, Roman A.

To date, special interest has been paid to composite scaffolds based on polymers enriched with hydroxyapatite (HA). However, the role of HA containing different trace elements such as silicate in the structure of a polymer scaffold has not yet been fully explored. Here, we report the potential use of silicate-containing hydroxyapatite (SiHA) microparticles and microparticle aggregates in the predominant range from 2.23 to 12.40 µm in combination with polycaprolactone (PCL) as a hybrid scaffold with randomly oriented and well-aligned microfibers for regeneration of bone tissue. Chemical and mechanical properties of the developed 3D scaffolds were investigated with XRD, FTIR, EDX and tensile testing. Furthermore, the internal structure and surface morphology of the scaffolds were analyzed using synchrotron X-ray µCT and SEM. Upon culturing human mesenchymal stem cells (hMSC) on PCL-SiHA scaffolds, we found that both SiHA inclusion and microfiber orientation affected cell adhesion. The best hMSCs viability was revealed at 10 day for the PCL-SiHA scaffolds with well-aligned structure (~82%). It is expected that novel hybrid scaffolds of ... mehr

Zugehörige Institution(en) am KIT Institut für Industrielle Informationstechnik (IIIT)
Laboratorium für Applikationen der Synchrotronstrahlung (LAS)
Institut für Angewandte Informatik und Formale Beschreibungsverfahren (AIFB)
Institut für Angewandte Informatik (IAI)
Institut für Photonenforschung und Synchrotronstrahlung (IPS)
Publikationstyp Zeitschriftenaufsatz
Jahr 2018
Sprache Englisch
Identifikator ISSN: 2045-2322
URN: urn:nbn:de:swb:90-837674
KITopen ID: 1000083767
HGF-Programm 56.03.30; LK 01
Erschienen in Scientific reports
Band 8
Heft 1
Seiten Article: 8907
Vorab online veröffentlicht am 11.06.2018
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