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Evaluation of click chemistry microarrays for immunosensing of alpha-fetoprotein (AFP)

Dadfar, Seyed Mohammad Mahdi; Sekula-Neuner, Sylwia; Trouillet, Vanessa; Liu, Hui-Yu; Kumar, Ravi; Powell, Annie K.; Hirtz, Michael

The level of cancer biomarkers in cells, tissues or body fluids can be used for the prediction of the presence of cancer or can even indicate the stage of the disease. Alpha-fetoprotein (AFP) is the most commonly used biomarker for early screening and diagnosis of hepatocellular carcinoma (HCC). Here, a combination of three techniques (click chemistry, the biotin–streptavidin–biotin sandwich strategy and the use of antigen–antibody interactions) were combined to implement a sensitive fluorescent immunosensor for AFP detection. Three types of functionalized glasses (dibenzocyclooctyne- (DBCO-), thiol- and epoxy-terminated surfaces) were biotinylated by employing the respective adequate click chemistry counterparts (biotin–thiol or biotin–azide for the first class, biotin–maleimide or biotin–DBCO for the second class and biotin–amine or biotin–thiol for the third class). The anti-AFP antibody was immobilized on the surfaces via a biotin–streptavidin–biotin sandwich technique. To evaluate the sensing performance of the differently prepared surfaces, fluorescently labeled AFP was spotted onto them via microchannel cantilever spotting (µCS). ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000104346
Veröffentlicht am 18.12.2019
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Angewandte Materialien - Energiespeichersysteme (IAM-ESS)
Institut für Anorganische Chemie (AOC)
Institut für Nanotechnologie (INT)
Karlsruhe Nano Micro Facility (KNMF)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2019
Sprache Englisch
Identifikator ISSN: 2190-4286
KITopen-ID: 1000104346
HGF-Programm 43.22.03 (POF III, LK 01) Printed Materials and Systems
Weitere HGF-Programme 49.01.01 (POF III, LK 02) INT-KNMF Structuring Lab
Erschienen in Beilstein journal of nanotechnology
Verlag Beilstein-Institut
Band 10
Seiten 2505–2515
Vorab online veröffentlicht am 16.12.2019
Schlagwörter ProposalID 2014-012-004310 DPN, XPS
Nachgewiesen in Web of Science
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