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Impact of incubation conditions on protein and C-Phycocyanin recovery from Arthrospira platensis post- pulsed electric field treatment

Akaberi, Sahar; Krust, Damaris; Müller, Georg; Frey, Wolfgang; Gusbeth, Christian

Abstract (englisch):
Pulsed electric field (PEF) was conducted for the extraction of proteins/C-Phycocyanins from Arthrospira platensis.
The cyanobacterial suspension was treated with 1 μs long pulses at an electric field strength of 40 kV·cm−1 and a treatment energy of 114 kJ·kgsus−1 and 56 kJ·kgsus−1. For benchmarking, additional biomass was processed by high pressure homogenization. Homogeneity of the suspension prior to PEF-treatment influenced the protein/C-phycocyanin extraction efficiency. Stability of C-Phycocyanin during post-PEF incubation time was affected by incubation temperature and pH of the external medium. Biomass concentration severely affect proteins/C-Phycocyanins extraction yield via PEF-treatment. The optimum conditions for extraction of proteins/ C-Phycocyanin was obtained at 23 °C while incubating in pH 8-buffer. The energy demand for PEF-Treatment amounts to 0.56 MJ·kgdw−1 when processing biomass at 100 gdw·kgsus−1. PEF treatment enhances the protein/CPhycocyanin extraction yield, thus, it can be suggested as preferential downstream processing method for the production of C-Phycocyanin from A. platensis biomass.



Originalveröffentlichung
DOI: 10.1016/j.biortech.2020.123099
Zugehörige Institution(en) am KIT Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 06.2020
Sprache Englisch
Identifikator ISSN: 0960-8524
KITopen-ID: 1000117787
HGF-Programm 35.13.01 (POF III, LK 01)
Biomassen und Vorbehandlung
Erschienen in Bioresource technology
Band 306
Seiten 123099
Schlagwörter Pulsed electric field treatment, Arthrospira platensis biomass, C-Phycocyanin, Protein Extraction efficiency
Nachgewiesen in Scopus
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