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Rapid response to pandemic threats: immunogenic epitope detection of pandemic pathogens for diagnostics and vaccine development using peptide microarrays

Heiss, Kirsten; Heidepriem, Jasmin; Fischer, Nico; Weber, Laura K.; Dahlke, Christine; Jaenisch, Thomas; Loeffler, Felix F.

Abstract:
Emergence and re-emergence of pathogens bearing the risk of becoming a pandemic threat are on the rise. Increased travel and trade, growing population density, changes in urbanization, and climate have a critical impact on infectious disease spread. Currently, the world is confronted with the emergence of a novel coronavirus SARS-CoV-$_{2}$, responsible for yet more than 800 000 deaths globally. Outbreaks caused by viruses, such as SARS-CoV-$_{2}$, HIV, Ebola, influenza, and Zika, have increased over the past decade, underlining the need for a rapid development of diagnostics and vaccines. Hence, the rational identification of biomarkers for diagnostic measures on the one hand, and antigenic targets for vaccine development on the other, are of utmost importance. Peptide microarrays can display large numbers of putative target proteins translated into overlapping linear (and cyclic) peptides for a multiplexed, high-throughput antibody analysis. This enabled for example the identification of discriminant/diagnostic epitopes in Zika or influenza and mapping epitope evolution in natural infections versus vaccinations. In this review, we highlight synthesis platforms that facilitate fast and flexible generation of high-density peptide microarrays. ... mehr

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Verlagsausgabe §
DOI: 10.5445/IR/1000123472
Veröffentlicht am 05.02.2021
Originalveröffentlichung
DOI: 10.1021/acs.jproteome.0c00484
Scopus
Zitationen: 5
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Zitationen: 7
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Mikrostrukturtechnik (IMT)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2020
Sprache Englisch
Identifikator ISSN: 1535-3893, 1535-3907
KITopen-ID: 1000123472
HGF-Programm 47.01.03 (POF III, LK 01) Biol.Netzwerke u.Synth.Regulat. IMT
Erschienen in Journal of proteome research
Verlag American Chemical Society (ACS)
Band 19
Heft 11
Seiten 4339-4354
Vorab online veröffentlicht am 05.09.2020
Schlagwörter infectious diseases, epitope mapping, microarrays, array synthesis technologies
Nachgewiesen in Web of Science
Scopus
Dimensions
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