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Biological signalling supports biotechnology – Pulsed electric fields extract a cell-death inducing factor from Chlorella vulgaris

Krust, Damaris; Gusbeth, Christian; Müller, Alexander S.K.; Scherer, Daniel; Müller, Georg; Frey, Wolfgang; Nick, Peter

Abstract:
Compared to mechanical extraction methods, pulsed electric field (PEF) treatment provides an energy-efficient and gentle alternative. However, the biological processes involved are poorly understood. The unicellular green microalga Chlorella vulgaris was used as model organism to investigate the effect of PEF treatment on biological cells. A viability assay using fluorescein diacetate measured by flow cytometry was established. The influence of developmental stage on viability could be shown in synchronised cultures when applying PEF treatment with very low specific energies where one part of cells undergoes cell death, and the other part stays viable after treatment. Reactive oxygen species generation after similar low-energy PEF treatment could be shown, indicating that PEFs could act as abiotic stress signal. Most importantly, a cell-death inducing factor could be extracted. A water-soluble extract derived from microalgae suspensions incubated for 24 h after PEF treatment caused the recipient microalgae to die, even though the recipient cells had not been subjected to PEF treatment directly. The working model assumes that low-energy PEF treatment induces programmed cell death in C. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000139980
Veröffentlicht am 12.11.2021
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Hochleistungsimpuls- und Mikrowellentechnik (IHM)
Botanisches Institut (BOTANIK)
Publikationstyp Zeitschriftenaufsatz
Publikationsmonat/-jahr 02.2022
Sprache Englisch
Identifikator ISSN: 1567-5394
KITopen-ID: 1000139980
HGF-Programm 38.05.01 (POF IV, LK 01) Anthropogenic Carbon Cycle
Erschienen in Bioelectrochemistry
Verlag Elsevier
Band 143
Seiten 107991
Projektinformation SABANA (EU, H2020, 727874)
Vorab online veröffentlicht am 30.10.2021
Nachgewiesen in Web of Science
Scopus
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