Large Volume Reconstruction of Pyrolyzed Origami Cellulose Structure by Automated Section Acquisition for Array Tomography

The video shows the large volume reconstruction of a pyrolyzed origami cellulose structure using automated section acquisition for Array Tomography. The sample was reconstructed from 1045 serial sections with a thickness of 200 nm, resulting in an analytical volume of 1050 µm x 410 µm x 209 µm. Light Microscopy (LM) is used on the complete set of serial sections to image the entire volume. Additionally, a smaller region of interest (ROI) is imaged by Electron Microscopy (EM) and reconstructed in higher detail to demonstrate the versatility of the imaging modalities. The video demonstrates the potential of this approach for analyzing complex materials.