KIT | KIT-Bibliothek | Impressum | Datenschutz

Micro simulated moving bed chromatography-mass spectrometry as a continuous on-line process analytical tool

Diehm, Juliane 1; Witting, Lennart 1; Kirschhöfer, Frank 1; Brenner-Weiß, Gerald 1; Franzreb, Matthias ORCID iD icon 1
1 Institut für Funktionelle Grenzflächen (IFG), Karlsruher Institut für Technologie (KIT)

Abstract:

Continuous manufacturing is becoming increasingly important in the (bio-)pharmaceutical industry, as more product can be produced in less time and at lower costs. In this context, there is a need for powerful continuous analytical tools. Many established off-line analytical methods, such as mass spectrometry (MS), are hardly considered for process analytical technology (PAT) applications in biopharmaceutical processes, as they are limited to at-line analysis due to the required sample preparation and the associated complexity, although they would provide a suitable technique for the assessment of a wide range of quality attributes. In this study, we investigated the applicability of a recently developed micro simulated moving bed chromatography system (µSMB) for continuous on-line sample preparation for MS. As a test case, we demonstrate the continuous on-line MS measurement of a protein solution (myoglobin) containing Tris buffer, which interferes with ESI-MS measurements, by continuously exchanging this buffer with a volatile ammonium acetate buffer suitable for MS measurements. The integration of the µSMB significantly increases MS sensitivity by removing over 98% of the buffer substances. ... mehr


Verlagsausgabe §
DOI: 10.5445/IR/1000164206
Veröffentlicht am 13.11.2023
Cover der Publikation
Zugehörige Institution(en) am KIT Institut für Funktionelle Grenzflächen (IFG)
Publikationstyp Zeitschriftenaufsatz
Publikationsjahr 2024
Sprache Englisch
Identifikator ISSN: 1618-2642, 1618-2650
KITopen-ID: 1000164206
HGF-Programm 43.33.11 (POF IV, LK 01) Adaptive and Bioinstructive Materials Systems
Erschienen in Analytical and Bioanalytical Chemistry
Verlag Springer-Verlag
Band 416
Seiten 373–386
Vorab online veröffentlicht am 10.11.2023
Nachgewiesen in Dimensions
Scopus
Web of Science
KIT – Die Forschungsuniversität in der Helmholtz-Gemeinschaft
KITopen Landing Page